TumorImagerTM

TumorImagerTM

TumorImager TM

The TumorImager is a first of its kind device designed to accurately and efficiently measure the size of subcutaneous xenograft tumor models in mice and rats. It is capable of measuring the volume of multiple, irregular and even necrotic tumors.

  • Volume data is more accurate and precise resulting in improved statistics (better p-values) and higher sensitivity
  • Measurements are more operator independent
  • The system does not require sedation of animals for the measurement process since scanning takes ~ 1 second
  • Users can also save scanned tumor profiles (but not color image) for further analysis
  • With the accompanying TumorImager program, researchers can acquire data, tabulate and display results, perform statistical analysis and export to many common formats

The sensor head with mount can be detached from the controller base unit and placed inside a laboratory hood to maximize available space. In order to ensure optimal ergonomics for each user, the sensor head can also be adjusted in multiple axes to reduce the risk of repetitive strain injury.

TumorImager TM - Rapidly scan subcutaneous xenograft tumors

The working principle

The TumorImager is an image acquisition and processing system that utilizes a laser/camera system to project a line over the animal being measured. The animal is held beneath the positioning mask so that the laser scans over it and the camera captures the data. It then creates a point cloud representing the x, y, and z coordinates defining the animal surface area. The z-axis information is obtained from the triangulation of the data points. Using patented algorithms, the tumor is then segmented and the volume calculated. The working principle is shown to the right.
Diagram for TumorImager TM - Rapidly scan subcutaneous xenograft tumors

Since it does not assume a predefined shape (as in the case of caliper based measurements), the TumorImager can measure the volume of any irregular shaped tumor, including necrotic tumors. Many animals develop necrotic tumors near the end of a study. Accurate measurements of these types of tumors can only be made with the TumorImager.

Please see the paper “A Structured Light-based System for Scanning Subcutaneous Tumors in Laboratory Animals by I. C. Girit et al, Comparative Medicine, Vol 58, No 3 , p264-270” for further information.

 

The measurement process

Samples from TumorImager TM - Rapidly scan subcutaneous xenograft tumors

For users, the measurement procedure with this new device is simple; in fact it is easier than using calipers. The process is:

  1. Pick up an animal and position it under the circular mask attached to the scanner head so that the tumor is in the center facing toward the scanner.
  2. Press the scan button on the computer screen or push the foot pedal if you wish to hold the mouse with two hands.
  3. After the laser line scans the tumor (<1.4 secs.) the mouse can be returned to its cage.
  4. The segmented tumor image together with the volume value is displayed on the program screen and it is ready for the next animal

This process takes less time than making caliper measurements.

 

TumorImagerTM Specifications

Measurement range, x axis 41 mm
Start of measurement range along x axis 2.5 mm (before the mask)
End of measurement range along x axis 37.5 mm (after the mask)
Camera working distance 100 mm
Maximum tumor size along x-y 25 mm
Maximum tumor height 20 mm
Tumor scan time ~1 s
Resolution, z axis 0.04% (10 µm)
Linearity, z axis (±3 sigma) 0.2% (50 µm)
Light source Laser diode 655 nm, 15 mW
Laser class Class 2M
Computer Interface Firewire and USB 2.0
Interface cable length Up to 6ft

 

System Requirements

  • Intel® i5 or better processor
  • Microsoft® Windows XP SP3, Vista SP2 or Windows 7,  8,  8.1
  • Minimum 4GB of RAM and 250MB of available hard-disk space for program installation
  • Minimum 1,280×1,024 monitor resolution video card with OpenGL capability
  • Internet or phone connection required for product activation, remote diagnostics and support
  • 1 USB and 1 Firewire port for the TumorImager and extra USB ports for balance, caliper and RFID readers, etc.
  • MS SQL Server 2008 R2, 2012 or 2014 database