TumorManager: Manage Subcutaneous Xenograft Tumor Study Data, and Calculate Tumor Volume


TumorManagerTM is a fully integrated program that allows scientists to manage every aspect of their subcutaneous xenograft tumor studies. Using an intuitive user friendly interface the program allows complete control of every step including:

  • Protocol design
  • Prestudy measurement and randomization
  • Dosing calculation
  • Automatic volume and weight data acquisition
  • Clinical observations
  • Powerful statistical analysis
  • Flexible report creation

Tumor Volume Calculation Problems Associated with Calipers

Caliper volume calculations are based on three assumptions. The shape of the tumor is assumed to be an ellipsoid. In most cases, the height of the tumor is assumed to be equal to its width. And the number pi is set to be 3, resulting in a tumor volume formula, V = 0.5 x L x W x W.  The first two assumptions are not fulfilled throughout tumor growth. At the beginning of tumor growth, tumor heights are much smaller than the tumor widths. And the shape of the tumors deviate away from ellipsoid at the later stage of their growth. These two assumptions, therefore, result in larger than real tumor volume estimates.

In addition to size, our system provides tumor volume calculation. Results of a study including this data are more accurate and more detailed.

A few of the problems with tumor size calculation and tumor volume calculation using calipers are summarized below:

Extensive involvement of human operators can cause transcription errors, either when recording the data in the laboratory during the measurement phase, or when transferring data to a spreadsheet. Protocols often change from one study to another, e.g. the number of animals, or the number of groups. Modifying spreadsheet templates to adapt to these new parameters can also cause additional errors.

Paper or Excel-based results are generally static; therefore, real-time information such as Tumor Doubling Time (TDT), Log Cell Kill (LCK), T/C etc is not available to the scientist. Using TumorManagerTM these important parameters are calculated and plotted after every measurement is made. Parameters such as TDT can be very useful to a researcher to monitor the progress of a study when they are available instantaneously.

Spreadsheet like programs typically have very limited statistical analysis capabilities. They do not provide ANOVA based multiple comparison analyses (e.g. Dunnett, Duncan, etc.), or Kaplan Maier survival analysis. Data from these spreadsheets need to be transferred to a commercial statistical analysis program for further analysis adding to the complexity and time requiTumorManager software program for tumor studiesred. The turnaround time can be even longer if the data must sent to a biostatistician for further analysis.

TumorManagerTM is a program designed to eliminate all of the above-mentioned problems. TumorManagerTM stores all data in a standard MS SQL Server database. Data inquiry and storage are validated. When each tumor size measurement is taken all information related to that data including the identity of experimenter, date and time of the measurement and the device used is recorded. TumorManagerTM protocols are defined by the study manager ensuring full user compliance even the nomenclature to be used.


Program Specifications:

  • Windows-based functionality using a mouse, touch screen, and foot pedals for program control.
  • Protocol driven functions such as data entry, dosing and task generation.
  • Program limits: 20 groups/study, 100 animals/group, 20 drugs/study.
  • Interfaces to calipers, serial balances and RFID readers plus data from other sources can be entered manually.
  • SQL Server 2005-2016 backend.
  • Secured program and database.
  • A utility to randomize animals to groups based on their ID, animal weight, tumor volume, and custom data.TumorManager software program for tumor studies
  • Study owner determined security rights for users.
  • Alerts for the maximum allowed tumor volume and animal weights.
  • 2D and 3D plots for Volume – Days after Inoculation, Volume – Animal Weight, TDT, TC, TGI, Log Cell Kill etc.
  • Extensive statistical analyses such as descriptive, ANOVA, homogeneity/normality tests, ANOVA based linear (e.g. Dunnett, Duncan) or nonlinear (e.g. Mann-Whitney, Kruskal Wallis) comparison tests, Kaplan-Meier Survival test.
  • Report generation for volume data, plots, and statistical analyses.
  • Reports can be exported to Word, Excel, HTML, and pdf.
  • FDA 21 CFR Part 11 Electronic Records and Signatures compliant.


System Requirements

  • Intel® I5 or better processorTumorManager software program for tumor studies
  • Microsoft® Windows XP with SP3, Vista SP2 or Windows 7, 8, 8.1
  • Minimum 4GB of RAM and 750MB of available hard-disk space for program installation
  • Minimum 1,280×1,024 monitor resolution
  • Internet or phone connection required for product activation, remote diagnostics, and support
  • 1 USB port for program updates
  • MS SQL Server 2005-2016 database